Imaging, learning and memory, neuronal plasticity

Optical imaging of new protein synthesis in living neurons and brain tissues
2014 Seed Grant

Wei Min, Ph.D.
Department of Chemistry
Columbia University

Protein metabolism plays a key regulatory role in the nervous system, in both health and disease. Learning and memory functions in particular require ongoing synthesis of new proteins. With a grant from Brain Research Foundation, researchers at Columbia University are learning more about this crucial and mysterious process.

The nervous system is a very busy place. Wei Min, Ph.D., and colleagues are trying to determine specifically how busy protein molecules are, and exactly how they function.

Dr. Min’s study is focused on overcoming technological barriers to observing protein synthesis, and learning more about cellular activity in the part of the brain, the hippocampus, that regulates short- and long-term memory.

Technology breakthrough

Extensive efforts have been made in the past to use a variety of imaging technologies to learn more about protein synthesis in the brain. All have fallen short.

For his study, Dr. Min proposed using a newly developed, but untested imaging technique to observe protein synthesis in newborn mice. If successful, the technique that he designed would facilitate an entirely new body of knowledge.

Highly revealing

His team is already able to directly observe, with unprecedented resolution, the dynamics of protein synthesis in living neurons. The detail able to be seen and documented includes space- and time-specific data that are crucial to understanding metabolic activity.

Although the study is still at an early stage, Dr. Min said the team is somewhat surprised by what they have so far observed. When complete, the study will yield new information about how the process occurs down to the level of fine dendritic structures in live neurons as well as in brain tissues.

Implications for many

Dr. Min expects this new imaging technique will help neuroscientists develop new insights relevant for multiple neurodegenerative diseases, including Alzheimer’s, Parkinson’s, and Huntington’s disease.

In addition, the technique may offer a way to study autism, which is known to be related to too much protein in specific types of cells. Dr. Min’s study may also give neuroscientists a way to better understand abnormal metabolism in brain tumors. This is the type of impactful research leading to more advancements that the BRF is eager to support.

2014 Fay/Frank Seed Grant recipient Wei Min, Ph.D., is an assistant professor in the department of chemistry at Columbia University, New York City. The goal of his research is to give neuroscientists a powerful tool to study the intricate dynamics of protein synthesis, which play a crucial role in regulating learning and memory.

Other Grants

Lindsay M. De Biase, Ph.D., University of California Los Angeles
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How the nervous system constructs internal models of the external world
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Xiaojing Gao, Ph.D., Stanford University
When Neural Circuits Meet Molecular Circuits: Quantitative Genetic Manipulation with Single-cell Consistency
Cells are the building blocks of our bodies. We get sick when the cells “misbehave”. The way modern gene therapies work is to introduce genes, fragments of DNA molecules that…
Rafiq Huda, Ph.D., Rutgers University
Conducting the orchestra of movement—functional role of striatal astrocytes in health and disease
Movement requires coordinated activity across a large brain-wide network. The striatum is a particularly important part of this circuit; it integrates motor-related information from many distinct brain regions to regulate…